【摘要】 用新生乳鼠心肌做原代细胞单层培养后均加入心得安（Propranolo）。分为4组:对照组;去甲肾上腺素（NE）组;NE+CEC组;NE+Nifedipine组。加入³H-胸腺嘧啶核苷后测放射性计数。结果表明,CEC阻断α_1b亚型受体后,不影响NE刺激心肌细胞DNA的合成;Nifedpine阻断α_1a亚型受体后,完全阻断了NE的作用。提示,α_1a亚型受体可能是交感-儿茶酚胺刺激心肌肥大形成的机理之一。更多还原

【Abstract】 Neonatal rat cardiac myocytes were monolayerly cultured in the DMEM medium. Forty-eightbours later the agonists and/or antagonists were added in a total volume of 500 ul, in the presence ofpropranolol. The experiments were divided into four groups: the control group; norepinephrine （NE）group; NE+CEC group; NE+Nif. group. After 24 hours the cells were harvested and the radio acti-vity （³H-TdR） was measured. The results showed that the CPM increased in the NE group comparedwith the control group （p<0.001）; the effect of NE was not influenced by CEC, but was totallyblocked by Nif. These suggested that the DNA synthesis in the myocardium cells stimulated by NEwas associated with α₁. adrenoceptor and without α_1b adrenoceptor.更多还原