【摘要】 <正> 核酶是新发现的一类基因调控RNA,它可以序列特异性地催化切割靶RNA。实际上,核酶是一种具有酶的特性的RNA分子。核酶主要是通过与靶RNA配对、切割而使其失去生物功能。由于核酶是在RNA水平上进行作用,有严格的序列特异性,长期使用对细胞无副作用,并且结构简单,可人工设计,因此很有希望用于基因治疗。目前,核酶已发展成为一门分子生物学技术,正被广泛地研究。更多还原

【Abstract】 Ribozyme is a kind of RNA which can catalyze the cleavage of the sequence-specific RNA. However,there have been few reports about the effect of ribozyme on HBV.We have developed the hammerhead ribozyme ( Ripc ) which aims at the pregenome RNA of HBV ayw at the 1796 site in the genome. The ribozyme efficiently cleaved its substrate in vitro. More than 75 percent of the substrate is cleaved after incubation with the ribozyme at 42℃ for 2 hours. The result suggests that ribozyme ai ming HBV genome may reduce gene expression of HBV in vivo.更多还原