【摘要】 人体17p等位基因探针PYNZ22对28例肠癌病人DNA作RFLPs分析,24例出现信息量(85％),其中18例有等位基因丢失。结果提示,采用PYNZ探针结合RFLPs方法,可能对肠癌的基因诊断具有潜在性临床应用价值。更多还原

【Abstract】 It has been demonstrated that loss of heterozygosity is a common genetic alterationin human colorectal carcinoma. In this study, the analysis of chromosome 11 and 17restriction fragment length polymorphisms (RFLPs) were performed on twenty-eightsets of tumor and non-tumor intestine tissue from patients with colorectal carcinoma.Eighteen(75%)of twenty-four informative samples showed allele loss in tumor tissuefor PYNZ22 (17p). No loss of heterozygosity was detected with the probe Ha-ras(11p).These results indicate that 17p allelic deletion might be a potential marker for colorectalcarcinoma and the loss of heterozygosity of 17p in Chinese colorectal carcinoma seemssimilar to that of the other human species in the world.更多还原