【摘要】 在22℃下莴苣（Lactuca sativa L.）种子萌发率达到70%左右时,25℃下的种子几乎不萌发。400ppm外源乙烯可使25℃下培养3天的种子萌发40—50%。在22℃下内源乙烯的释放量比25℃下明显地大。Ca²⁺专一性螯合剂乙二醇双乙胺醚NN四乙酸（EGTA）、Ca²⁺通道抑制剂La³⁺和Co²⁺、钙调素（CaM）拮抗剂氯丙嗪（CPZ）不但抑制22℃下的种子萌发,也明显抑制25℃下乙烯诱导的萌发。但La³⁺和CPZ不降低内源乙烯的释放。表明Ca²⁺和CaM在乙烯诱导的反应过程中发挥一定的作用,而与乙烯的生物合成无关。Co²⁺不但抑制内源乙烯的生成,还影响乙烯的诱导反应过程。更多还原

【Abstract】 Relevant effects of ethylene and Ca²⁺ on germination of lettuce （lactuce sative L.） seeds were investigated. It was shown previously that lettuce seeds were highly sensitive to temperatures. More than 70% of seeds germinated at 22℃, but they ceased to germinate at 25℃. 40%—50% of seeds could be induced to germinate after imbibition with 400 ppm exogenous ethylene for 3 days at 25℃. The amounts of endogenous ethylene liberated at 22℃ were much greater than those at 25℃. Ethyleneglycol bis NN tetraacetic acid(EGTA, Ca²⁺ specified chelating regent) La³⁺, Co²⁺ and chlorpromazin（CPZ, calmodulin antagonist） could be used not only to inhibit germination at 22℃, but also to inhibit germination induced by ethylene at 25℃. Although La³⁺ and CPZ inhibited seed germination, they could not repress the production of ethylene at 22℃. It was suggested that Ca²⁺ and CaM affected the induction response of ethylene to lettuce seed germination, but had no effect on ethylene liberation. Co²⁺ could be applied to inhibit the action as well as its production of ethylene.更多还原