【摘要】 取一雌株美味猕猴桃(Actinidia deliciosa line No.26)当年生枝条的茎段再生苗的叶片、幼嫩茎段及叶柄分别在培养基上诱导愈伤组织。上述3种不同愈伤组织来源的原生质体经培养后,叶片及茎段愈伤组织来源的原生质体再生大量小植株。在1987—1989年间,再生小植株通过移栽或嫁接,有76株叶片愈伤组织及21株茎段愈伤组织来源的原生质体再生植株定植成活。叶片愈伤组织来源的原生质体再生植株中的3株,在1991年5月开花。其中2株是雌株,1株是雄株。经人工授粉后,雌株已结不少果实。从而证实,美味猕猴桃的体细胞再生植株存在性别分化。再生植株的叶片、节间及花的形态上出现大量的体细胞无性系变异。鉴定了16株再生植株染色体,其染色体数每株不一样,变动在116—180之间。更多还原

【Abstract】 Protoplasts were isolated from leaf, petiole and stem segment-derived calli induced from one pistillate plant of Actinidia deliciosa line No. 26. Regenerated plantlets were obtained from protoplasts of leaf-derived and stem segment-derived calli, while only calli regenerated from protoplasts of petiole-derived calli. Seventy-six plants from protoplasts of leaf-derived calli and 21 plants from that of stem segment-derived calli survived after transplanting or grafting during 1987—1989. One staminate plant and two pistillate plants bloomed in May, 1991 and fruited soon afterwards. In all of those three plants regenerated from protoplasts of leaf-derived calli, sex differentiation occurred from somatic cells of Actinidia was verified. Soma-clonal variation on leaf shape and plant morphology was obviously appeared. Chromosome number identified from 16 plants varied from 116 to 180.更多还原