【摘要】 用酶联免疫吸附测定和胶体金免疫电镜定位技术对三种钙调素抗体与植物和动物钙调素的免疫反应特性进行了比较研究。结果表明,在与小麦钙调素的相对亲和力中,抗小麦钙调素抗体大于抗 DNP 修饰猪脑钙调素抗体,抗牛脑钙调素抗体则很弱。抗小麦钙调素抗体与小麦钙调素的 K_D（解离常数）值为2.50×10^-9mol/L;抗 DNP 修饰猪脑钙调素抗体与小麦钙调素的 K_D 值为2.82×10^-8mol/L,而它与牛脑钙调素的 K_D 值为1.90×10^-mol/L。定位玉米根尖细胞钙调素,抗小麦钙调素抗体比抗 DNP 修饰猪脑钙调素抗体有更高的标记密度.定量小麦钙调素,抗小麦钙调素抗体比抗 DNP 修饰猪脑钙调素抗体有较高的检测灵敏度。更多还原

【Abstract】 The immunoreactivities of three rabbit anticalmodulin antibodies with plant and animal calmodulin（CaM）were tested using the techniques of ELISA and immuno-gold electron mi- croscopy.The results showed that the relative affinity of anti-wheat calmodulin antibody（W- Ab）was greater than that of anti-dintrobenzene-modified porcine brain CaM antibody（D-Ab）, and the relative affinity of anti-bovine brain CaM antibody（B-Ab）for wheat CaM was weak The dissociation constants（K_D）of CaM antibody and CaM measured by ELISA were:2.50× 10^-9 mol/L for W-Ab/wheat-CaM,2.82×10^-8 mol/L for D-Ab/wheat CaM,and 1.90×10^- mol/L for D-Ab/bovine brain-CaM.The affinity of B-Ab for wheat CaM was so low that ELISA detection of the K_D value was not feasible.Localization of CaM of corn root-tip cells with immuno-gold electron microscopic technique revealed that using the W-Ab probe,the la- belled CaM density was greater than using the D-Ab probe,and with B-Ab probe,the density was very low.In quantitation of wheat CaM by competitive ELISA,the detection sensitivity of W-Ab for wheat CaM was higher than that of D-Ab for wheat CaM.These results suggest that the affinity of anti-plant CaM antibody for plant GaM is higher than the affinity of anti-animal CaM antibody for plant CaM.Using anti-plant CaM antibody in the study of plant CaM could raise the sensitivity of immunological detection for plant CaM.The difference in cross-reaction of anti-plant GaM antibodies and anti-animal CaM antibodies with animal CaM and plant CaM was discussed.更多还原