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菊花切花“黄秀凤”茎尖培养加速繁殖的研究

An Investigation on Rapid Propagation of Chrysanthemum morifolium by Means of Shood-tip Cultivation

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【作者】 孙景欣冯志萍

【Author】 Sun Jingxin Feng Zhiping Department of Biology, Zhongshan University

【机构】 中山大学生物学系中国科学院华南植物研究所

【摘要】 本文报道菊花切花“黄秀凤”茎尖培养和加速繁殖。诱导愈伤组织培养基为MS+NAA0.2-1;分化培养基为MS+NAA0.2-1+KT0.2-1;芽的增殖培养基为MS+GA31-6+BA0.1-1。研究结果表明,从一块外植体每年可获4.096×1015至5.31×1017株试管苗。移植大田后,成活率达95%以上。

【Abstract】 Chrysanthemum moriforlium shoot tip culture and rapid propagation were reported, The shoot tips were cultured on MS medium containing naphehaleneacetic acid (NAA) 0.2~1.0mg/1 for callus induction, NAA+Kinetin (all between 0.2~1.0mg/1) for shoot differentiation and Gibberellic acid (GA3) (1.0~6.0mg/1)+6-benzylaminopurine (6-BA)(0.1~1.0mg/1) for shoot multiplication. The results showed that 4.096×1015 to 5.310×1017 plantlets can be produced from just one parent shoot tip per year. The ratio of survival upon transplantion is about 95 percent.

【关键词】 菊花茎尖培养繁殖
【Key words】 Chrysanthemum morifoliumshoot tip culturerapid propagation
  • 【文献出处】 中山大学学报论丛 ,Sun Yatsen University Forum , 编辑部邮箱 ,1992年03期
  • 【被引频次】2
  • 【下载频次】48
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