【摘要】 本文探讨用MTT法进行人实体瘤抗癌药敏试验的条件和影响因素,结果表明该法之最适肿瘤细胞浓度为0.5—1×10~5个/孔,最适培养时间为三天.167例实体瘤的八种抗癌药物敏感试验结果说明其与肿瘤细胞生长特性、病理类型和肿瘤标本取材部位等因素有关.MTT法有操作简单、重复性好、成功率较高、便于推广等优点更多还原

【Abstract】 We studied the methodology of 3-(4, 5-dimethyl-2-lhiazoly) )2, 5-diphenyl-2H-tetrazolium bromide(MTT) colorimetric assay for anticancer drug sensitivity test of human solid tumors. Results showed that the optimal cell concentration per well is 0.5-1 × 10~5, the optimal incubation period for the action of anticancer drugs on tumor cells is three days. The concentration of anticancer drugs used should be varied with different drugs. We observed 167 samples of human solid tumors. The applicability of this test is concerned with the growth characteristics of tumor cells, histological types of malignancy, and the resected portion of the tumor specimen. The MTT colorimetric assay is a simple, rapid and reliable method for anticancer drug sensitivity test of human solid tumors更多还原