【摘要】 作者用自己建立的只需一只小鼠即可作GABA受体分析的方法(NSB、TB、SB:CV<10%),进行了肝性脑病(HE)小鼠大脑的GABA受体分析.此外,测定了HE时GABA调节蛋白的变化.结果表明HE组虽有Triton处理的突触膜上低亲和力GABA受体消失,但起作用的GABA受体位点不变.同时,新鲜和处理突触膜上GABA受体亲和力增加,提示GABA突触传递活性增强.HE小鼠突触膜上结合的GABA调节蛋白含量减少,抑制活性降低.它可能是HE小鼠GABA受体亲和力增加的原因之一更多还原

【Abstract】 The GABA hypothesis of hepatic encephalopathy (H.E) was supported by many reports with regard to its content, metabolism, permeability against blood brain barrier, etc. But the experimental data which reflect the changes of GABA recep-ptors in H.E. were limitted only to rabbits and rats.We established a method with a small concentration of membrane protein (50ug/ 0.2ml) for the analysis of GABA receptors with satisfactory results (NSB, TB, SB: CV<10%; SB/TB: 66-30% at the concentrations of 3H-GABA 11,28-225.5 pmo-le/L). The synaptic membrane prepared from the brain of a single mouse was sufficient for an experiment, thus, avoiding the pooling of brain extracts from several mice.Our results showed for the first time that mice with H.E were associated with a loss of low-affinity receptors in Triton treated synaptic membrane, but the densities of the active binding sites were not changed. The increase of the affinities of GABA receptors in fresh and Triton treated synaptic membrane suggests the increase of GABA synaptic transmissional activity in mice with H.E. Boin the amount of protein extracted from the fresh membrane and its inhibitory activities as GABA-modulin of mice, with H.E. were significantly lower than those of the cont-rol(p<0.01) . These may explain partly for the increase of the affinities of GABA receptors in mice with H.E更多还原