【摘要】 本实验用酸性α醋酸萘脂酶染色法观察实验感染旋毛虫小鼠外周血T淋巴细胞及其亚群的变化,以斑点-ELISA试验观察血清IgG抗体的变化。小鼠感染后第3d起外周血T淋巴细胞开始增高,第14d达最高水平,此后开始降低,第77d仍未降至正常水平,而其中斑点型细胞(辅助性T细胞,T_h)和弥散型细胞(抑制性T细胞,T_s)呈反向变化,前者减少,后者增多,T_h/T_s比值降低,表明旋毛虫感染过程中宿主免疫功能受抑制。同时观察到感染后第7d外周血清IgG抗体滴度开始增高,第28-42d达最高水平,第140d仍维持较高水平。更多还原

【Abstract】 Acid α-mphthyl esiere.se (ANAE),a cytoplasmic marker,was used to identify the T lymphocytes and their subpopulations in peripheral blood of mice infected with Trichinella spiralis,and Dot-ELISA was used to identify the serum IgG antibody of the infected mice. The results showed that T lymphocytes increased on the d3 after infection,reaching the peak on d14,and then remainel in number greater than normal up to d77.The spotted granular ANAE positive cells (Help T cells,Th) deereased and the scattered granular ANAE positive cells (Suppresor T cells,Ts) increased,leading to a drop of the Th/Ts ratio.The reduction in host immune function during T.spiralis infection might be related to the drop of Th/Ts ratio.At the same time,the serum IgG antibody increased on d7 after infection,reaching the peak on d28,and remained higher up to d140.Tl:e results indicated that the host’s cellular immunological level was lower while the host’s IgG antibody level was higher during T.spiralis infection.更多还原