【摘要】 用超声粉碎马来丝虫微丝蚴断片抗原间接荧光抗体试验,于1988～1989年对丝虫病不同流行区人群进行监测,能相应揭示高低不同的阳性率,阐明流行程度和防治效果。在已消灭丝虫病的上海监测,1:10滴度阳性率仅2.5%,而在不同流行区的安徽和江苏,1:10滴度阳性率分别为24.9%与19.3%。表明本法可用于马来和班氏丝虫病流行区的流行病学调查和监测。更多还原

【Abstract】 Indirect immunofluorescent antibody test(IFA)using sonicated microfilariae of B.malayi asantigen has been carried out to diagnose filariasis since 1983,The microfilariae were first sonicated into frag-ments 20—30 μm long,and then applied to Alum—Gelatin coated slides.Specific fluorescence was seen atthe cut ends of the fragmented microfilariae and along the cuticle.Comparison of results obtained with seracollected by venepuncture and eluates of ear lobe blood on filtter paper showed no statistical difference.Hence,the latter could be used instead of the former.In field investigation,when the same titer was considered as positive,the increase in positive rate amongpopulation coincided with the positive rate of microfilaraemia and the variation of positive rates ran parallelwith the degree of endemicity.It is concluded that the sonicated microfilarial antigen might be useful for theepidemiological investigation and surveillance of filariasis.更多还原