【摘要】 用大剂量大黄复制成Wistar大鼠中医脾虚模型。血浆和脾脏中cAMP下降,cGMP上升,cAMP/cGMP比值下降,溶菌酶(Lys)活性不变,脾脏cAMP-磷酸二酯酶(cAMP-PDE)活性不变;腺苷酸环化酶(AC)活性下降。结果提示大黄复制中医脾虚模型所致的免疫功能下降与环核苷酸系统有关,环核苷酸及其比值变化是脾虚症的参考指标,但对于反应非特异性免疫功能指标之一的Lys活性无明显影响。更多还原

【Abstract】 A model relevant to spleen insufficiency syndrome of Chinese traditional medical practice was established by feeding rats with large doses of Rhubarb. The model showed a decrease of cAMP, increase of cGMP with a lowered cAMP/cGMP ratio in their serum and spleen. Adenylcyclase (AC) activity was also lowered, but lysozymal (Lys) and cAMP-phosphodiesterase (cAMP-PDE) activities remained unchanged. The results showed that changes of cyclic nucleotides level and ratio of cAMP/cGMP were important targets of spleen insufficiency and that immunodepression by Rhubarb was related with cyclic nucleotide system. Lys plays an important role in non-specific immunity, but the results also indicated that Rhubarb does not inhibit Lys activity.更多还原