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小儿牛黄散有效成分的HPLC定量

HPLC Determination of Active Constituents in Xiaoerniuhuang Powder

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【作者】 史林于洪建聂万达

【Author】 Shi Lin, Yu Hongjian, (Tianjin Lerentang Medicinal Factory, Tianjin, 300122)

【机构】 天津乐仁堂制药厂天津乐仁堂制药厂 300122300122

【摘要】 用反相HPLC法测定小儿牛黄散中小檗碱、巴马汀、大黄素、绿原酸的含量。用甲醇提取散剂样品,在ODS柱上,用外标法在各自特征吸收处测定。此法具有分离好、灵敏度高、重现性强、操作简单等优点。

【Abstract】 The reversed-phase high-performance chromatography was developed for determing the content of berberine, palmatine, emodin and chlorogenic acid in Xiaoerniuhuang Powder on ODS column. The powder samples extracted with methanol were determined separatly at their particular absorption points using external standard method.This method had advantages of perfect separatin, high sensitivity, good reproducibility and simple operation.

  • 【文献出处】 中成药 ,Chinese Traditional Patent Medicine , 编辑部邮箱 ,1992年02期
  • 【被引频次】6
  • 【下载频次】32
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