【摘要】 本文报告了采用聚合酶链反应（即PCR技术）检测人宫颈癌中人乳头瘤病毒16型（HPV16）的E₆/E₇相关序列。在正链引物（SP）和反链引物（ASP）的诱导下,以二步温控法聚合酶链反应对34例人宫颈癌组织DNA进行检测,发现宫颈癌中HPV16E₆/E₇相关序列存在的阳性率达67.6％（23/34）。本研究结果提示:HPV16可能在宫颈癌的发生发展过程中起重要作用,采用PCR技术选择性地检测HPV16的转化基因（E₆、E₇）对深入探讨宫颈癌发生的机理、早期发现宫颈癌患者等有重要意义。更多还原

【Abstract】 Detection of the sequence related to E₆/E₇ of human papillomavirus type16 in Chinese cervical cancers using polymerase chain reaction （PCR） has beenreported in this paper. We have used the two-step protocol PCR to detectDNA sequence related to HPV16 E₆/E₇ in 34 cervical cancer tissues, by primingwith SP and ASP, the positive percentage of HPV 16 E₆/E₇ in cervical cancerswas 67.6% （23/34）. This result showed that HPV 16 may play an importantrole in development and progression of cervical cancers, and use of the PCRtechnique for selective detecting the transforming genes（E₆/E₇） of HPV16 maybe helpful with research of cervical carcinogenesis and early finding of cervicalcancer patients.更多还原