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甜橙成年树侧芽离体繁殖诱导成苗研究

A STUDY OF THE DIRECT PROPAGATION IN VITRO WITH LATERAL BUDS OF ADULT TREES OF CITRUS SINENSIS OSBECK

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【作者】 林伯年胡春根沈德绪张君圻

【Author】 Lin Bonian, Hu Chungen, and Shen Dexu ( Department of Horticulture, Zhejiang Agricultural University, Hangzhou 310029 )Zhang Juiqi ( Zhejiang Institute of Subtropical Crop, Wenzhou 325005 )

【机构】 浙江农业大学园艺系浙江亚热带作物研究所 杭州 310029杭州 310029温州 325005

【摘要】 对甜橙(Citrus sinensis(L.)Osbeck)成年树的侧芽离体培养进行了研究。试验采用综合指标A值,兼顾存活率、增殖率和质量3个因素,筛选出适宜甜橙侧芽增殖的激素配比;离体新梢茎段的继代增殖须降低激素的浓度。不同甜橙品种对培养基反应不同,存活率、增殖率、质量级别和A值均有显著差异。二步生根法可获得满意生根率。

【Abstract】 The lateral buds of adult trees ( Citrus sinensis Osbeck ) cultured in vitro were studied. The synthetic quota A, involving three factors of survival percentage, propagation percentage and quality grade, was used in this experiment. The optimum hormone proportion for the propagation of orange lateral buds was selected. The hormone concentration should be lowered for the subculture of the shoots in vitro.Different varieties of orange are with different response to the same culture media, and with significant different survival percentage, propagation percentage, quality grade and A value. The result showed that the satisfying rate of rooting was obtained with the use of the method of two-step.

【关键词】 甜橙成年树离体繁殖
【Key words】 Citrus sinensisAdult treeIn vitro
  • 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,1992年03期
  • 【被引频次】36
  • 【下载频次】68
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