【摘要】 胆固醇能在开路下吸附在悬汞电极的表面,并在电位扫描过程中发生氧化。在0.1 mol/LK₂HPO₄—KH₂PO₄缓冲溶液（pH 7.8）中,差示脉冲溶出峰电位在-0.08V,溶出电流与胆固醇浓度在10^-7～10^-6mol/L范围内呈线性关系。经5min富集,检测下限达8×10^-8mol/L。本文对胆固醇在电极上的吸附、氧化机理,测定条件和干扰情况进行了探讨,并提出了直接测定血清中游离胆固醇的方法。更多还原

【Abstract】 Cholesterol can be adsorbed on the surface of the static mercury drop electrode, and then oxidized during anodic potential scan. The stripping peak potential in differential pulsevoltammetry is at-0.08 V in 0. 1 mol/L K₂HPO₄--KH₂PO₄ buffer solution. The stripping current islinear with the concentration of cholesterol in the range of 10^-7～10^-6 mol/L. The detection limitfound is 8×10^-8 mol/L after a 5 min preconcentration period. The adsorption and oxidation mechani-sms of cholesterol at the mercury electrode were been explored. The experimental conditions for mea-suring free cholesterol in human sera were examined.更多还原