【摘要】 在Mg²⁺存在下.用血小板膜与(γ—³²P)ATP保温,以观察川芎嗪对³²P掺入磷脂和蛋白质的影响,结果表明:血小板膜中存在磷脂酰肌醇（PI）激酶和磷脂酰肌醇—4—磷酸（PIP）激酶,而胞浆中缺乏或极少;ATP促进肌醇磷脂磷酸化;川芎嗪抑制血小板中PIP和20K蛋白质的磷酸化.半数抑制浓度分别为40μmol·L^-1和110μmol·L更多还原

【Abstract】 Thrombocytic membranes were incubated with [μ-32P] ATP in the presence of Mg2+ to observe the effect of tetramethylpyrazine on incorporation of 32 P into phospholipids and proteins. The result showed there are phosphatidylinositol kinase and phosphatidylinositol 4 - phosphate kinase in platelet membranes, while almost none or very little in cytosol. ATP stimulated the phosphory-lation of phosphoinositides. Tetram-ethylpyrazine inhibited the phosphoryla-tion of phosphatidylinositol 4-phosphate and 20K protein in platelets. Concentrations giving 50% inhibition (IC50) were 40 μmol · L-1 and 110 μmol · L-1 respectively. The data might be valuable in clarifying the antiplatelt mechanism of tetramethylpyrazine.更多还原