【摘要】 应用胶质银技术对正常食管鳞状上皮、单纯增生、非典型增生、原位癌和浸润癌石蜡切片进行核仁组成区蛋白定量研究。发现随病变进展,核仁形成区蛋白计数明显递增,其大小、形态、分布也明显改变。核仁形成区蛋白均值除Ⅱ、Ⅲ级非典型增生间无显著性差异外(P>0.2),其余各病变组间均有显著与高度显著性差异(P<0.05,P<0.001)。表明核仁形成区蛋白定量可鉴别癌及癌前病变,并为食管脱落细胞学诊断奠定了组织学基础。更多还原

【Abstract】 Using silver colloid staining technigue, AgNOR were investigated with quantitative method on paraffin sections of normal esophagel sqamous epithelium, simple hyperplasia, dysplasia, carcinoma in situ and invasive carcinoma.The results showed that AgNOR enumeration were obvious increased and AgNOR size, shape and distribution pattern wer obvious changed along with the development of esophageal lesioos.A significant difference was found in the mean of AgNOR enumeration between various groups (P<0.001, P<0.01) except the grade and grade dysplasia (P>0.2).It indicated that AgNOR could be used as quantitative parameter for the differentiation of esophageal carcinoma from precancerous lesions and for laying the histological foundation of esophageal exfoliative diagnosis.更多还原