【摘要】 <正> 病毒性肝炎给人类造成的危害是众所周知的。传统的分类法将肝炎病毒分为甲型、乙型、非甲非乙型和丁型四类。近年发现,非甲非乙型病毒(NANBHV)有两种类型:一类经血液传播(致输血后肝炎),另一类经肠道传播,1989年,Choo和Rcycs等分别将二者克隆,并命名为丙肝病毒(HCV)和戊肝病毒(HEV)。因此,新命名法特别是在1989年9月东京会议正式命名法确定后,将肝炎病毒分为HAV、HBV、HCV、HDV、HEV。在世界范围内,HEV肝炎流行多发地见于中国(特别是新疆)、缅甸、墨西哥、更多还原

【Abstract】 The specific probe for entericall transmitted HEV RNA was prepared first by applying PCR with one pair of specific primers (16 bp) on cDNA converted from HEV RNA using cDNA synthesis kit, and then by labeling with nonradioactivc Dig-11-dUTP. ET-HEV RNA was purified from the fecal samples collected from the acute phage HEV patients during the epidemic of 1986—1987 in Xinjiang. The results of Dot-blot indicated that the probe was of high specificity and high sensitivity (at least to 0.5 pg) for the target RNA. This was the first report world-wide of the specific probe prepared for ET-HEV RNA. Further study was being made on such probe.更多还原