【摘要】 <正> 有关中华猕猴桃蛋白酶(Actinidin,E C 3、4.22.14)催化功能基团的研究表明:CYSH—25、His—161为酶活性部位必需基团,而Lys和Trp残基为酶活力表现所必需。本文中报道应用水溶性碳二亚胺(EDC)修饰动力学方法,进一步探讨羧基更多还原

【Abstract】 Actinidin was isolated and purified from fruits of wild Actinidia chinensis. The enzyme can be repidly inactivated by water-soluble carbodiimide (1-ethy-3-(3-dimethyl aminoprgpyl) carbodi-imide, EDC). The chemical modification kinetic data indicated that only one carboxyl group was related to the enzyme activity.更多还原