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利用气相色谱法同时测定土壤中反硝化作用和固氮作用的强度
SIMULTANEOUS DETERMINATION OF INTENSITY OF THE DENITRIFICATION AND NITROGEN FIXATION IN SOIL BY GAS CHROMATOGRAPHY
【摘要】 土壤中反硝化菌和固氮菌的作用强度与其产生的N2O及C2H2还原成C2H4的量有一定关系。本文报导了为测定土壤中微量的N2O、C2H2和C2H2数量,所建立的双柱双检测器一次进样同时测定的二维气相色谱法。此法能简单、快速、准确地反映出土壤中反硝化菌和固氮菌的作用强度。文中对仪器装置、色谱条件、样品处理、干扰物的消除、最小检测浓度、重复性、方法应用等进行了报导。
【Abstract】 In this paper,a simultaneous determination of trace amount of N2O and CH4/C2H2 produced by the denitrification and nitrogen fixation in soil by two dimensional gas chromatography of dual column and dual detector was presented. A simple, fast and accurate analytical method was developed. Instrumental installation, column, conditions, sample disposal, interfering component elimination, minimum detectable concentration, repeatability and applications cf the method were reported.
【关键词】 气相色谱法;
反硝化菌;
固氮菌;
作用强度;
二维气相色谱法;
【Key words】 Gas Chromatography; Denitrobacteria; Azotobacteria; Action Intensity; Two Dimensional Gas chromatography;
【Key words】 Gas Chromatography; Denitrobacteria; Azotobacteria; Action Intensity; Two Dimensional Gas chromatography;
- 【文献出处】 微生物学杂志 ,Journal of Microbiology , 编辑部邮箱 ,1992年02期
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