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溶脲脲原体12个血清型标准株与6个地方分离株主要蛋白组份的分析

ANALYSIS OF MAJOR PROTEINS OF THE TWELVE STANDARD SEROVARS AND SIX LOCAL ISOLATES OF UREAPLASMA UREALYTICUM

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【作者】 叶元康殷常红王悦冶陆德源

【Author】 Ye Yuankang Yin Changhong Wang Yueye Lu Deyuan (Department of Microbiology, Shanghai Second Medical Univeraity, 200025)

【机构】 上海第二医科大学微生物学教研室上海第二医科大学微生物学教研室 200025200025200025

【摘要】 经SDS—PAGE和薄层扫描分析,18株溶脲脲原体有从分子量19—175KD 20多条蛋白区带。主要蛋白组份分布在32—140KD范围之间。其中32、52和94KD蛋白组份各株间差别较大,而其余主要蛋白组份各株间较类似。按蛋白组份的不同可将溶脲脲原体分为A、B两群。^群包括2、5、7、9、10、11、12血清型标准株和U_C、U_D、U_E、U_F地方分离株。B群包括1、3、6、13、14和U_A、U_B株。仅用SDS—PAGE方法分析溶脲脲原体蛋白组份不能区别菌株间的血清型。

【Abstract】 More than 20 protein bands with molecular weights of 19—175 KD in 18 strains of Ureaplosma urealyticum were detected by SDS-PAGE and thin layer scanning analysis. The molecular weights of major proteins were between 32 KD and 140 KD. Among them, the percentages of 67, 78 and 140 KD proteins were similar in all strains, but those of 32, 43 and 94 KD proteins were greatly different. Thus, Ureaplasma urealyticum can be divided into two groups: group A including serovars 2,5,7,9,10,11,12, and local isolates C,D, E, F; group B including serovars 1,3,6,13,14, and local isolates A, B. The serovars of Ureaplasma urealyticum could not be distinguished by using SDS-PAGE alone.

【关键词】 溶脲脲原体SDS-PAGE
【Key words】 Ureaplasma urealyticumSDS-PAGE.
  • 【文献出处】 微生物学通报 ,Microbiology , 编辑部邮箱 ,1992年05期
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