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苜蓿花叶病毒沉降系数的分析
THE ANALYSIS ON SEDIMENTATION COEFFICIENT OF ALFALFA MOSAIC VIRUS
【摘要】 苜蓿花叶病毒是一种多组分病毒,提纯的病毒样品中有五种不同形态的颗粒。在白三叶草分离物中的苜猪花叶病毒的鉴别研究中,使用超速离心分析法进行了该病毒的理化特性分析。在分析测试中我们使用了Schlieren光路与紫外吸收光路对照,二次变速法,配合适当的样品浓度和离子强度。获得了较为理想的可供分析计算的五个样品组分的扫描记录图,并得到了该五种组分颗粒的沉降系数S值,分别为S?=98s S?=82s S?=72s S?=65s S?=36s。
【Abstract】 The physical and chemical properties of alfalfa mosaic virus from white clover have been studied by analytical ultra-centrifuge. The results showed that this virus sample contains five different components which possess the S20 values of 98s, 82s, 72s, 65s, and 36s respectively. While measured the S values we have used schlieren light system by using UV system as control. The twice changed speed method also has been used to measure the S values at different concentration of samples under different ion strength.
【Key words】 Analytical ultra-centrifuge method; Change speed; Sedimentation cofficient; AMV;
- 【文献出处】 微生物学通报 ,Microbiology , 编辑部邮箱 ,1992年01期
- 【被引频次】2
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