【摘要】 本文通过高效液相色谱分析、热重分析、紫外分析和³¹P 核磁共振分析证明,磷酸二氢钠在常规180℃两小时干烤去热原处理过程中,约有9.2%缩合为二代焦磷酸钠。缩合磷酸盐所配成的缓冲液消光系数明显增大,从而导致生物制品在高效液相色谱纯度检定过程中出现异峰。更多还原

【Abstract】 Phosphate buffer is widely used as a solvent for bio-products and the phosphate salt isusually dry heated before use in order to remove pyrogen.By means of HPLC,heating-weight analy-sis,UV spectrum and ³¹PNMR analysis,we proved that NaH₂PO₄ will condense into Na₂H₂P₂O₂ andthe ratio was about 9.2% if it was heated at 180℃ in solid state for two hours.We also found thatthis caused the increase of absorption coefficient and a confusable peak in determination of purity of abio-product containing phosphate buffer by HPLC.更多还原