【摘要】 研究了竹红菌乙素（以下简称乙素,HB）对小鼠腹水型肝癌细胞（AH）的杀伤作用,并初步解释了其杀伤机理。实验证明乙素对AH细胞杀伤与乙素浓度的平方根成正比。乙素杀伤细胞的能力与竹红菌甲素（以下简称甲素,HA）相等,比血卟啉强,但比原卟啉弱,电镜观察可见:损伤细胞的膜失去连续结构,线粒体,内质网等细胞器变形,最终导致细胞空泡化。此外,细胞变形,表面微绒毛精细结构丧失,也是光敏损伤的主要特征。造成细胞死亡的原因包括¹O,O₂^-和·OH在内的活性氧,而·OH主要由O₂^-转换而来。更多还原

【Abstract】 In this paper, we primarily studied the HB-sensitized cytotoxicity to murine ascitic hepatoma cell (AH). The results showed that the cell mortality was roughly proportional to the square root of HB concentration. The ability to kill AH cell among hypocrellin A (HA) , HB, protoporphyrin (PP) , and he-motoporphyrin (HP) are in the following order: PP>HB>HA>HP. The ultr-astructure observation using TEM showed that the plasma membranes, mit-ochodria and endoplasmic recticulus were probably the main targets of photose-nsitization. After 10 minutes irradiation in the presence of HB, obvious ves-iculation and dramatic rupture of plasmic membranes were observed, but the nucleic membranes and nuclei did not change significantly. SEM revealed that the shape of the photodamaged cells changed obviously and the microvilli on the cells disappeared. By using active oxygen quenchers, spin trapping and spin silence techniques, we proved that singlet oxygens, superoxide anion radicals and hydroxyl radicals were involved in the process of HB-photosensitized cytotoxi-city.更多还原