【摘要】 <正> 目前,对胚状体发生过程中的生理生化研究表明,这一过程伴随有核酸、蛋白质等大分子物质合成速度的增加及与胚胎发生有关的特异性蛋白的合成;一些同工酶,如过氧化物酶、脂酶、细胞色素氧化酶和谷氨酸脱氢酶更多还原

【Abstract】 Embryogenesis was induced at highfrequency from suspension cultures of em-bryogenic calli derived from hypocotyl seg-ments of Medicago sativa L..An efficientseparation of embryoids at different deve-lopment stages was carried out using sucro-se density gradient centrifugation.Thechanges of three isoenzyme patterns at diffe-rent stages of embryoids were analysed.Itshowed that the isoenzyme patterns of pe-roxidase and cytochrom oxidase were diffe-rent.A specific band was observed onisoeasterase pattern of embryogenic calli.更多还原