【摘要】 <正> 3β,20α-羟基甾体脱氢酶(3β,20α-HSD)是和胚胎的发育密切相关的;我们于1987年首次从胎羊血中得到该酶的纯品,并对其有关性质进行了研究。但是繁杂的测酶活方法常常延误研究工作的顺利进行,因此寻求一种简便有效的测定方法是很有意义的。经多次实验,我们成功地将高效液相色谱技术(HPLC)用于3β,20α-HSD的活性测定,大大缩短了测定时间,促进了有关研究工作的顺利进行。本文将介绍这种新的测酶活方法。更多还原

【Abstract】 A high performance liquid chromatographic method for the determination of the 3β,20α-hydroxysteroid dehy-drogenase activity has been established. The assay proce-dure includes sample preparation, chromatographic separation and data acquisition based on the HPLC system. The advantages of HPLC method over the isotope method for determining the conversion of progesterone to 4-preg-nen-20α-ol-3-one are higher accuracy, rapidity and reproducibility.and avoidance of radioactive hazards.更多还原