Protoplasts were isolated from petioles of sterile seedlings 9f Orychophragmus violaceus. The medium for protoplast culture was Nitsch containing 100mg/L serine, 800mg/L glutamine, 13% sucrose and complemented with 0.5mg/L BA, 0.5mg/L NAA and 1mg/L 2,4-D(or with 0.5mg/L BA and 2mg/L 2,4-D). Protoplasts were cultured at a density of 2×10~5/ml. The frequency of protoplast division was about 40% after 7 days of culture. On MS medium containing 3mg/L BA and 0.05mg/L NAA, the protoplast-derived calli could regen...
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