【摘要】 B.subtilis中编码β-1,3-1,4葡聚糖酶的基因bglS,它的产物在E.coli中的合成受宿主,载体和基因片段插入方向的影响。从E.coli细胞中BglS蛋白质定域分析和SDS-PAGE中活性酶分子检测发现存在着32kD和27kD两种活性酶分子,酶分子在周质空间很少停留。这种不同的活性酶分子的出现和分泌特点,可能与大肠杆菌细胞蛋白质加工和转位的方式有关。更多还原

【Abstract】 A β-1,3-1,4-glucanase is encoded by bglS gene from B. subtilis. The synthesis of the gene product BglS in E. coli is affected by various hosts, different vectors and orientation of the gene fragment.By protein localization analysis and zymogram analysis of active enzyme molecules, we have found that the enzyme has two active molecules (32 kD and 27 kD) in E. coli and the amount of the enzyme in the periplasmic space is very small. The presence of different active products and the specificity of secretion pattern in E. coli may be due to the difference between E. coil and B. subtilis in the protein processing and translocation.更多还原