【摘要】 以sprague-Dawley大鼠为实验对象,研究胰岛素对大鼠肝脏及小肠apoAⅠ、CⅡ和CⅢ mRNA相对含量的影响。RNA斑点杂交技术分析表明,注射胰岛素后12h,大鼠肝脏和小肠apoAⅠ和CⅡ以及肝脏apoCⅢ mRNA相对含量均显著下降,小肠apoCⅢ mRNA相对含量无变化。更多还原

【Abstract】 Sprague-Dawley rats were used to study the effects of insulin on theapo AⅠ,CⅡ and CⅢ gene expressions. Using ³²P-labelled apo AⅠ, CⅡ and CⅢ recombinant DNA as probes, we determined the mRNA amounts of apo AⅠ, CⅡ and CⅢ in the liver and intestine of rat injected with insulin （1.5U/Kg weight of rat） by dot blot hybridization method. After injecting insulin, the mRNA amounts of apo AⅠ in liver and intestine were obviously decreased, to a lowest level （42%） at 6 h interval and restored to that of control group at 24h interval. Also, after injecting insulin, the mRNA amounts of apo CⅡ in liver and intestine were declined remarkably, to a lowest level （57%） at 12h interval, still lower than that of the control group （79%） at 24h interval. But the mRNA amounts of apo CⅢ showed no significant changes after injecting insulin both in liver and intestine when compared with the control group （P>0.05）.The experiment was repeated. 12h after injecting insulin, the mRNA amounts of apo AⅠ, CⅡ and CⅢ in liver and apo AⅠ and CⅡ in intestine were obviously decreased （45%, 51% and 54%, 35% and 37%, respectively, P<0.01, n=5） and the mRNA amounts of apo CⅢ in intestine remained unchange （P>0.05, n=5）. These results revealed that insulin could evidently affect the gene expression of apolipoproteins.更多还原