【摘要】 本文采用ELISA技术,实验研究了LPF-ELISA和FHA-ELISA的最适条件以及LPF和FHA免疫原在纯化过程中的动态。实验结果表明,LPF-ELISA的HP最佳包被浓度为0.413μg/100μl;所用第二抗体(抗LPF兔血清)的最佳浓度为2.8EU/100μl。FHA-ELISA的抗FHA豚鼠血清的最佳包被浓度为0.05EU/100μl,所用第二抗体(抗FHA兔血清)的最佳浓度为2.25EU/100μl。LPF和FHA在经培养、盐析、浸提、超速和分级离心后,其ELISA单位与蛋白量密切相关,呈同步消长。证明用LPF-ELISA和FHA-ELISA作新菌苗批量生产工序中的质量控制,是一种行之有效的方法。更多还原

【Abstract】 We have studied the optimal conditions for LPF-ELISA, FHA-ELISA and concentration variations of LPF and FHA in their purification processes. Our results indicated that tke optimal coating concentration of HP in LPF-ELISA was 0.413 μg/ 100μl and that of anti-FHA guinea-pig serum in FHA-ELISA, 0.05EU/100μl. The optimal concentration of second antibody in LPF-ELISA was 2.8EU/100μl and that in FHA-ELISA, 2.25 EU/100μl. After a series of purification processes of LPF and FHA,such as incubating, salting,soaking and differential centrifugation, the ELISA units of LPF and FHA were closely positively associated with the amount of protein. We concluded that it was practicable to use LPF-ELISA and FHA-ELISA ’aa a criterion for the quality control in the manufacture of vaccine.更多还原