【摘要】 对牦牛副伤寒牛病沙门氏菌（S. morbificans bovis）的染色体DNA进行提取,用基因工程中的“鸟枪法”,将限制性内切酶部分酶切染色体DNA片段,重组到载体pBR₃₂₂的Barn H I酶切位点上,再转化到大肠杆菌DH_5α中,经耐药表型筛选,在约1900个转化菌中找出了86个具有重组质粒的细菌株,经ELISA法检测,其中pS9,pS31表现出较强的表达,通过动物免疫试验,小鼠获5/8保护,豚鼠获6/8保护,大肠杆菌DH_5α无保护力（0/4,0/8）,证明本试验所克隆的片段是保护性抗原,为今后构建基因工程苗提供了依据。更多还原

【Abstract】 After extracting the chromosome DNA of S. morbificans, some fragments of chronlosome DNA obtained by festrictive endonuclease have been recombined to Bam H I site of carrier pBR₃₂₂, by means of "fowling piece test", then pBR₃₂₂ has been transferred into E. coli DH_5α. Screening test of drug resistance showed that 86 of 1900 treated cells possessed the recombination plasmid. The ELISA test showed that pS9 and pS31 possessed the fairly good expression. Immune tests on experimental animals showed that 5/8 of mice and 6/8 of quinea pigs aquired protection, but E. coli. DH_5α（0/8 and 0/4）had not any protection. These results indicated that the cloning fragment in this study belong to genome of protective antigen, and it has supplied a good base for genetic engineering vaccine in future.更多还原