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兔输精管吻合术后精子腺苷脱氨酶活性的变化
THE VARIATION OF ADENOSINE DEAMINASE ACTIVITY ON RABBIT SPERM MEMBRANE AFTER VASO-VASOSTOMY
【作者】
凌晓阳;
孟建平;
张洪艳;
董春娥;
王中山;
李杨;
赵丹;
赵雪俭;
【Author】
LING Xiao-Yang, et al Jilin Research Institute for Family Planning, Changchun. Although the function of spermatogenesis of the testis could recover after vaso- vasostomy, spermacrasia and lack of fertilizasion ability of the sperm happened in some cases.In this study, an animal model of vasovasostomy with Japanese white rabbit was established and the sperm plasma membrane bound adenosine deaminase (ADA)activity was analyzed.The result showed that the ADA activity (186.87±63.73 nMols/mg Pro/h for non-pregnant group, 132.81±49.85 nMols/mg Pro/h for pregnant group) was significantly higher than that of control (81.22±47.63 nMols/mg Po/h) (P<0.01).Also a significant difference of the ADA activity between non-pregnant group and pregnant group was observed (P<0.01). These results were consistent with our previous investigation and we concluded that ADA is invloved in the process of sperm maturation.
【机构】
吉林省计划生育科研所;
吉林省计划生育科研所;
白求恩医科大学;
【摘要】 我们利用日本大耳白兔建立的输精管结扎1年吻合术5个月后的动物模型,分析了精子膜上腺嘌呤核苷脱氨酶(ADA)的活性以及该类精子膜上活性与受精能力之间的关系。实验结果显示吻合术5个月后,不孕组精子膜上ADA活性(186.87+63.73nMols/mgPro/h)明显高于孕组的ADA活性(132.81+49.85nMol/mgPro/h(P<0.01)。同时,两实验组ADA活性又高于对照组ADA活性(81.22+47.63nMol/mgPro/h)(P<0.01)。结果显示输精管结扎吻合后,精子膜上ADA活性明显升高是造成精子受精能力低下的原因之一。同时提示生殖道的病理变化将引致精子膜成分的变化。更多还原
【Abstract】 vasostomy, adenosine deamianse, sperm,更多还原
【基金】 国家计划生育委员会资助
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【文献出处】
男性学杂志
,
编辑部邮箱
,1992年04期
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