【摘要】 通过暗处理消耗螺旋藻细胞内积存的可供放氢的能源,然后外加不同的呼吸基质。了解到处于暗饥饿状态的螺旋藻可以利用不同基质维持放氢。蔗糖等基质可使放氢活性恢复到暗饥饿前充足照光时的水平,乙酰基和酮戊二酸的恢复效果较差。藻细胞利用不同基质支持的放氢活性还受反应系统中氧浓度的影响,当氧浓度超过10%时,放氢活性差不多被完全抑制。在不同的基质存在和光照条件下,由藻细胞提取得的ATP 浓度有一定差异,这种差异与放氢活性似乎有一定的对应关系,表明蓝藻的放氢活性在适宜放氢的条件下,可能还受藻细胞内ATP 水平的影响。更多还原

【Abstract】 Aftor consuming out of the inside energy That may be used for H₂ evolution in Spirulin　cell by dark hungry treatment,the algal cells could evolve hydrogen gas effectively by making useof respiratory materials such as sucrose,glucose,fructose and pyruvate.Some materials mayresume the whole hydrogen evolution activity to the control under light.The activity was affectedby concentration of oxygen in the reaction system.10% or more oxygen may completely inhibitedthe activity brought by extra materials.The results also showed that the hydrogen evolution activityunder different conditions may be related to the level of cellular ATP conentration.更多还原