节点文献
疏水吸附法分离纯化α—淀粉酶
Separation and Purification of α-Amylase from Fermentation Broth by Hydrophobic Adsorption
【摘要】 本文报道了一种从发酵液中分离纯化α—淀粉酶的新方法。α—淀粉酶发酵液用两种高分子量的絮凝剂澄清,滤液用一种国产吸附树脂吸附,然后用40%乙醇洗脱,所得酶粉的活性约为60000U/g。产率64.22%。
【Abstract】 A new method of separation of α-Amylase from fermentation broths is reported. , The α-amylase fermentation broth is clarified by two kinds of high molecular agglomerating agent and the filtrate is hydrophobically adsorbed by adsorptional resin made in China and then deadsorbed by 40% ethanol. The activity of the obtained enzyme powder is 60000 U/g and the yield is 64. 22%。
- 【文献出处】 离子交换与吸附 ,Ion Exchange and Adsorption , 编辑部邮箱 ,1992年04期
- 【被引频次】6
- 【下载频次】298