【摘要】 以魔芋葡甘聚糖为原料合成了葡甘聚糖凝胶(GM)和它的衍生物DEAE—GM弱碱性阴离子交换树脂。GM凝胶具有较好的碱、酸和热稳定性;具有分子筛作用;能把单糖和大分子多糖分开,能除去蛋白质制剂中的盐类。DEAE—GM的全交换容量是2.92—3.23mmol/g树脂,工作交换容量是40.8—42.2mg(牛血红蛋白)/ml树脂;对牛血清白蛋白的吸附量是15.76mg/ml树脂,并可以把羊红血球超氧化物岐化酶粗酶制剂分离成几个组份。此外,吸附固定化环状糊精葡基转移酶活性达3023.5u/g树脂。更多还原

【Abstract】 This paper describes the preparation and adsorbent properties of the Glucomannan Gel (GM) and its derivatives DEAE-GM anion exchanger. The GM gel is stable to base, acid,and heat. It can serve as molecular sieve in separating monosaccharide from polysaccharide, and in desalting proteins. The total capacity of DEAE-GM is 2. 92-3. 23 mmol/g and availabe capacity is 40. 8-42. 2mg (bovine haemoglobin)/ml. The adsorption capacify of bovine serum albumin is 15. 76mg/ml. The adsorption activity of the immobilized cyclodextrin glycosyltransferase by the DEAE-GM is 3023. 5u/g. In addition, DEAE-GM can separate the crude superoxide dismutase (SOD) (prepared from goat red cells) into several components.更多还原