【摘要】 本文探讨了样品处理方法对大鼠肌肉组织腺苷酸、乳酸和丙酮酸测定值的影响。结果表明:肌肉取出后迅速在液氮中夹冻并研磨成粉,在4℃用玻璃匀浆器匀浆能较完全地破碎细胞膜,得到满意的测定值。再经超声波处理,线粒体等亚细胞结构完全破碎,ATP和总腺苷酸(TAN)的测定值增加20％,乳酸和丙酮酸变化不大。超声波处理在1min以内AMP和ADP变化不明显,时间过长则随时间而升高。更多还原

【Abstract】 An ideal assay of adenine nucleotides, lactate and pyruvate in the extract of rat freeze-clamped muscle was achieved. Electromicroscopy showed that the cell membrane was destroyed by powdering the muscle in liquid N₂ and then homogenizing for 1 min. An additien of ultrasonic treatment for 1 min could thoroughly break the subcellular structures and lead to a remarkable increase in ATP and total adenine nucleotides with a little change of lactate and pyruvate. The prolongation of the treatment for two more minutes, however, could result in the breakdown of ATP.更多还原