【摘要】 以α-淀粉酶盐酸胍变性作用为对照,利用紫外差光谱、荧光光谱和圆二色谱法研究了α-淀粉酶、脱钙α-淀粉酶和牛血清白蛋白碳酸胍及碳酸钠的变性作用。光谱数据所反映的酶分子构象变化和酶活性的变化表明,碳酸胍阴离子CO₃^2-与酶蛋白间存在着复杂的作用,可能是碳酸胍强变性作用的重要原因之一。更多还原

【Abstract】 The comparative studies of the denaturation of α-amlase, Ca²⁺-free amylase and bovine serum albumin （BSA） by guanidine hydrochloride （GuHCl）, guanidine carbonate （Gu₂H₂CO₃） and sodium carbonate （Na₂CO₃） were carried out by means of UV difference, fluorescence and CD spectra. The analysis of the spectra data shows that the anion CO₃^2- of Gu₂H₂CO₃ might have some direct interaction with the molecules of α-amylase, which is one of the important factors leading to the great increase in the ability of Gu₂H₂CO₃ for the denaturation of the enzyme. The possible sites of the interactions on the enzyme molecules are described.更多还原