【摘要】 以人正常卵巢皮质层及手术切除的卵巢癌组织为材料,分别分离纯化细胞膜,测定膜蛋白的腺苷酸环化酶(AC)活性。结果:正常卵巢细胞膜AC活性为67±8U/mg蛋白;卵巢癌细胞膜AC活性明显高于正常,平均活性为234±86U/mg蛋白;卵巢癌中粘液性囊腺癌细胞膜AC活性高于浆液性囊腺癌者。更多还原

【Abstract】 It has been documented thatthe binding activity sites of human cho-rionic gonadotropin（HCG） receptor and kdin human ovarian tumors are differentfrom those in the normal ovary. Andsome observations suggest that the HCGreceptor depends on adenylate cyclase（AC）for its physiological functions. We studiedthe activity of AC in normal human ovaryand ovarian tumors. Five human ovarian specimens andeighteen ovarian tumor specimens wereobtained from women patients undergoinggynecological surgery. Ovaries were homo-genized and sonicated. The homogenateswere centrifuged at 1000×g for 15 min. Af-tersucrose density gradient ultracentrifuga-tion （78000×g, 4h）. the membrane fractionwas collected from interface between 33%and 37%. The membrane protein approxi-mately 10μg, ATP 1mmol/L, ³H-ATP5×10⁴cpm, sulphydrylethyl alcohol 10 mmol/L, ina final volume of 200μl of Tris-HCl buffer（50 mmol/L）, pH7.5, containing MgSO₄ 5mmol/L, were incubated at 35℃ for 10 min.The reaction was stopped by boiling water bath for 3 min. The AC activity （U/mg）: of humannormal ovary, 67±8, of cystadenocarcinomaserous, 146±70; of cystadenocarinoma mu-cinous, 289±83.更多还原