【摘要】 研究并总结了三氟甲磺酸(TFMSA)~*对8种肽-树脂化合物的裂解效率。以 TFMSA/TFA/DMS/对甲酚、TFMSA/TFA/对甲酚为“低”、“高”及“低-高”等条件的切肽试剂,用茚三酮定量分析的方法考察了 C-端残基、肽链长度、树脂连接结构、反应温度及反应时间等诸多因素对裂解反应完全度的影响。证明 TFMSA 在许多情况下可以代替 HF 的切肽试剂。更多还原

【Abstract】 The efficiency of TFMSA as a cleaving reagent for peptide-resin(Ⅰ-Ⅷ)was evaluated from the peptide substitution degree(SD)on resin(mmol/g)by quantitative ninhydrin test.Different situations,such as variations of C-terminal residue,resin linkage,the length of pep-tide chain,temperature and reaction time,etc,proved that TFMSA can be used very convenientlyas cleaving reagent substituting for HF in manycases.更多还原