【摘要】 试验于1991年在华南农业大学实验乳牛场进行,初步成功地获得乳牛雌性控制率达到81.8％的国内外先进水平,计有十头乳牛产犊:二雄、九雌,其中一头产异性双椟,研究试验结果包括三个方面:一是研制成具有一定的纯度和工作效价的H-Y抗血清;二是这种H-Y抗血清IgG确实存在有抑制牛Y染色体精子的基因抗体;二是掌握H-Y抗血精IgG与牛精液发生“感作”和导致正常受胎的技术方法。试验应用免疫遗传学原理,进一步证明H-Y抗血清IgG有抑制牛Y染色体精于的受精能力而获得较高的雌性控制率,具有很大的生物科学与经济价值更多还原

【Abstract】 This trial was proceeded in the experimental dairy farm at South China Agricultural University in 1991 and the primary coefficient of female control was successfully reached to 81.8%at an advanced level between the internal and outside the country.As a result of the trial,11 calves were born by 10 cows,including a twin of sexuallity at birth.It is of immense values to biological science and economy for this trial.It was found that it is possible for realizing of sexing sperm by treating of bovine semen with H-y antiserum IgG.It was also an evidence of using Density Gradient Centrifugation of Sucrose solution and Fluorescent Staining for inspectation of the effectiveness of H-Y antiserum IgG.We observed that the H-Y antiserum IgG showed marked effect of inhibition on Y-bearing sperm,so that we can achieve a good result of sexing control in dairy cattle.更多还原