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超氧化物歧化酶的氯甘脲和氯胺T放射性碘标记法的比较
A comparative study on radioiodine labelling of superoxide dismutase with iodogen and chloramine T
【摘要】 本文介绍超氧化物歧化酶(SOD)的氯甘脲和氯胺T标记方法的比较,结果表明:SOD用氯甘脲标记法131I标记率(55.34%)较同样条件下氯胺T标记法131I标记率(42.37%)为高;而且用氯甘脲标记方法的SOD酶的活性(0.5 U/μg)较氯胺T标记方法的SOD酶活性(0.2U/μg)要高。且前者具有操作简单、经济、高效、快速的优点。
【Abstract】 A comparison of radioiodine labelling methods of SOD with iodogen and chloramine T is reported. The result indicated that the radioiodine labelling rate for the former (55.34%) was higher than that for the latter (42.37%) at the same condition, and the enzyme activity for the former (0.5U/μg) was also higher than that for the latter (0.2U/μg). And the former is a simple, economic, efficient and speedy method.
【关键词】 超氧化物歧化酶;
氯甘脲;
放射性碘标记;
氯胺T;
【Key words】 Superoxide dismutase Iodogen Radioiodine labelling Chloramine T;
【Key words】 Superoxide dismutase Iodogen Radioiodine labelling Chloramine T;
- 【文献出处】 核技术 ,Nuclear Techniques , 编辑部邮箱 ,1992年02期
- 【被引频次】1
- 【下载频次】65