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超氧化物歧化酶的氯甘脲和氯胺T放射性碘标记法的比较

A comparative study on radioiodine labelling of superoxide dismutase with iodogen and chloramine T

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【作者】 孙爱敏

【Author】 Sun Aimin(Institute of Radiation Medicine, Shandong Medical Academy, Jinan 250002)

【机构】 山东省医学科学院放射医学研究所 济南 250001

【摘要】 本文介绍超氧化物歧化酶(SOD)的氯甘脲和氯胺T标记方法的比较,结果表明:SOD用氯甘脲标记法131I标记率(55.34%)较同样条件下氯胺T标记法131I标记率(42.37%)为高;而且用氯甘脲标记方法的SOD酶的活性(0.5 U/μg)较氯胺T标记方法的SOD酶活性(0.2U/μg)要高。且前者具有操作简单、经济、高效、快速的优点。

【Abstract】 A comparison of radioiodine labelling methods of SOD with iodogen and chloramine T is reported. The result indicated that the radioiodine labelling rate for the former (55.34%) was higher than that for the latter (42.37%) at the same condition, and the enzyme activity for the former (0.5U/μg) was also higher than that for the latter (0.2U/μg). And the former is a simple, economic, efficient and speedy method.

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