【摘要】 建立了用于电子显微镜生物样品制备的接触式低温固定法中的传热过程模型,并求得了一维常物性条件下的精确解。讨论了样品内部冷却速率的一些变化规律及其与样品初始温度和固化温度的关系,为实际的低温固定操作提供了理论指导。更多还原

【Abstract】 The heat transfer process in impact cryofixation of bio-specimen preparation for election microscopy is analysized theoretically. An exact solution of the local cooling rate is presented for one-dimensional system with the constant thermal properties. The effects of the initial temperature and melting temperature of bio-specimens on the cooling rate are treated of. The results prove admirably useful in the practice of cryofixaion.更多还原