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应用等速电泳法测定转移因子中ATP、c-AMP的含量

Isovelocity Phoresis Measuring ATP,cAMP of Transfer Factor

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【作者】 李竹朱光奇陈荣善

【Author】 Li Zhu~1 Zhu Guangqi~2 Chen Rongshan (~1 Institute of Identification of Medicine and Biological Products of China; ~2 Department of Epidemiology,~3 Department of Microbiology,Hubei Medical College,Wuhan 430071,China)

【机构】 中国药品生物制品鉴定所湖北医学院流行病学教研室湖北医学院微生物学教研室

【摘要】 应用等速电泳(IP)法检测了以人的脾脏、扁桃腺、白细胞为原料生产的四批转移因子(TF)中的 ATP 和 c—AMP 的含量,经多次复测,重复性好,变异系数均小于5%,适用于常规分析。

【Abstract】 ATP and cAMP of 4 batches of transfer factor (TF) purified from human being’s spleen,tonsil and WBC were measured by isovelocity phoresis (IP) method.After many repetitions,the results were similar.All the variation coefficients were <5%.We suggest that IP method could be as an analysis routine for measuring ATP and cAMP of TF.

【关键词】 转移因子/分离与提纯电泳
【Key words】 transfer factor/IPelectrophoresis
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