【摘要】 以番茄叶片的染色体DNA为模板,用PCR技术扩增ACC合成酶基因,得到了2.5kb的特异性扩增片段,并将该片段克隆到了pUC18的KpnI和BamHI位点上。重组子的酶切图谱和插入片段两端的序列分析结果与Rottmann等报道的一致。更多还原

【Abstract】 A 2.5kb specific fragment was obtained by PCR amplification of ACC synthase gene from total genomie DNA of tomato leaves, and cloned into pUC18 digested with KpnI and BamHI. The restriction maps of reeombinants and the sequence of both ends of the insert were identical to the results reported byRottmann et al..更多还原