【摘要】 构建了枯草杆菌表达载体,并在枯草杆菌细胞中首次实现了HBeAg的高效表达,为乙肝的早期诊断和疫苗生产开辟了一条新的途径。更多还原

【Abstract】 The tac promoter from plasmid pDR540 DNA was inserted into BamH Ⅰ /Hind Ⅲ site in Bacillus subtilis plasmid pNQ122 to get expression vector pNQ122-tac. HBV-e antigen and antibody are the most important ruler for inspecting type B hepatitis. Therefore, the e gene, which is included in c gene, from HBV DNA by Bgl Ⅱ was cloned into pNQ122-tac in BamHl site. It expressed high level HBeAg with biological function in Bacillus subtilis. This is a new development in the early diagnosis of type B hepatitis and the production of hepatitis vaccine.更多还原