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不同品种金鱼草多倍体诱变方法的研究

POLYPLOID MUTATION OF VARIOUS COMMON SNAPDRAGON BREEDS

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【作者】 岳桦任俐王雁

【Author】 Yue Hua Ren Li Wang Yan(Northeast Forestry University)

【机构】 东北林业大学东北林业大学

【摘要】 用秋水仙素水溶液对3个不同品种金鱼草种子进行多倍体诱变,设计采用18种处理方法进行试验。研究结果:不同品种的诱变剂适宜浓度范围不同,红色及矮生品种适于低浓度处理。白色品种则诱变剂范围较宽。筛选出红色品种以0.05%,48 h最好,诱变率为36%;白色品种以0.2%,24 h最好,诱变率为17%,矮生品种为0.03%,96 h最好,诱变率为22%。对各种处理变异苗存活数目的动态变化进行观察、分析其原因。发现不同品种金鱼草的气孔大小不同,其矮生品种气孔较小,加倍植株均比二倍体植株叶绿体数多1.5—2.5倍。

【Abstract】 Experiments on polyploid mutation of 3 kinds of common snapdragon breeds were carried out by colchicine solution treatments in this paper, and 18 kinds treatment method were tested. The results show that: Different mutagen are suitable for different concentration limits; Red and short-stalk breeds are suitable for low concentration treatment but wider mutagen limit for white breed;The best treatment for red breeds is 0.05% and 48 hours, 0.2% and 24 hours for white one and 0.02% and 96 hours for short-stalk one; The mutation ratios are 36%, 17% and 22% respectively.And the chloroplast number of the polyploid plants was 1.5-2.5 times more than that of the diploid plant by observing the stomata apparatus size and the numbers of guard cell and chloroplast of three common snapdragon breeds.

  • 【文献出处】 东北林业大学学报 ,Journal of Northeast Forestry University , 编辑部邮箱 ,1992年04期
  • 【分类号】Q943
  • 【被引频次】26
  • 【下载频次】181
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