【摘要】 灵芝子实体经热水提取,乙醇分级沉淀,除游离蛋白质和小分子杂质,得到具有免疫调节作用及抗氧自由基活性的多糖GLA。进一步分离纯化,得到8个多糖均一体,对其中主要成分GLA₄、GLA₇、GLSP_1～35个多糖均一体进行了化学结构研究。以完全酸水解、过碘酸盐氧化、Smith降解、光谱测定及气相色谱分析等证明,GLA₄、GLA₇为以葡萄糖为主的中性杂多糖,GLSP₁为鼠李半乳聚糖肽,GLSP₂及GLSP₃为葡聚糖肽。它们的精甙键类型均以β-（1→3）（1→6）及（1→4）为主,GLA₄尚有α-甙键存在。更多还原

【Abstract】 The polysaccharide GLA was obtaiaed from the fruit-body of Ganoderma lucidum through the ext-raction of hot water, fractional precipitation with ethanol, cleavage of the free protein and removal ofmicromolecular impurities. Immune regulatory function and SOD activity were fouad in GLA. Furtherisolation and purification yielded eight homogenous polysaccharides, five of them, CLA₄, GLA₇, GLSP_1-3 accordingly, underwent structural studies. Through the procedure of total acidic hydrolysis, perio-date oxidation, Smith degradation, spectral determination and GC analysis, GLA₄ and GLA₇ were sho-wn to be heteropolysaccharides containig mainly glucose. GLSP₁ to be a rhamnogalactan peptide. whileGLSP₂ and GLSP₃ to be glycan peptide.β-（1→3）（1→6）and（1→4）linkage are the main type of bond ex-isting in the molecules and α-bond is also existed in GLA₄.更多还原