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水稻黄矮病毒的纯化和病毒蛋白质及RNA组分的分析
PARTIAL PURIFICATION OF RICE YELLOW STUNT VIRUS AND CHARACTERIZATION OF VIRUS STRUCTURAL PROTEINS AND RNA GENOME
【摘要】 从人工接种水稻黄矮病毒(Rice yellow stunt virus,RYSV)的水稻茎杆中提取到部分纯化的RYSV制品。分析了RYSV的蛋白质组分,表明它的结构蛋白含有L(170k)、G(84k)、N(60k)、M1(31k)、M2(29.5k)和一个分子量为42k的蛋白,并测定了G蛋白氨基末端的部分氨基酸序列。经测定,RYSV RNA的分子量约为12kb。
【Abstract】 Partially purified RYSV preparation was obtained by extraction from stalks of artificially infected rice plants. Analysis of RYSV structural proteins by SDS-PAGE revealed that it consisted of 5 protein species, L(l70k), G(84k), N(60k), Ml(3lk)and M2 ( 29.5k ),in addition to a protein with molecular weight of 42k. Furthermore, the N terminal amino acid sequence of 15 residues for G protein was determined by automated Edman degradation method.The size of RYSV RNA genome was estimated to be about 12 kb.
【关键词】 植物弹状病毒;
水稻黄矮病毒;
病毒结构蛋白;
病毒RNA;
【Key words】 Plant Rhdbdoviruses Rice yellow stunt virusViral structural proteins Viral RNA;
【Key words】 Plant Rhdbdoviruses Rice yellow stunt virusViral structural proteins Viral RNA;
- 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,1992年01期
- 【被引频次】7
- 【下载频次】123