【摘要】 用BALB/C小鼠作为动物模型,应用放射免疫分析法对烧伤后早期小鼠腹腔液申的前列腺素E₂（PGE₂）进行动态测定,同时检测小鼠脾淋巴细胞对刀豆蛋白A刺激的反应性.结果表明,烧伤后7d内各期的PGE₂水平均明显高于正常对照值（67.2±36.8nmol/L,P<0.05～0.001）;同时脾淋巴细胞对刀豆蛋白A刺激的反应性在烧伤后明显降低（P<0.05～0.001）.体外实验也表明,烧伤鼠腹腔液提取物对正常鼠的淋巴细胞转化功能有抑制作用,与未烧伤鼠的腹腔液提取物的作用相比,具有明显差异（P<0.05）.提示烧伤后体内的PGE₂生成明显增多,而且对淋巴细胞转化功能具有一定程度的抑制作用.说明烧伤后早期体内PGE₂的增高是介导免疫功能抑制的因素之一.更多还原

【Abstract】 Using the BALB/C mice as an animal model, the kinetic changes in the production of Prostaglandin E₂（PGE₂） in the peritoneal fluids of the mice during the early stage of burns, were determined by means of radioimmunoassay. The ConA-induced ³H-thymidine incorporation in the mice’ s splenocytes were examined simultaneously. A series of in vitro tests of PGE₂ were carried out with the extracts obtained from peritoneal fluid of both burned and unburned mice. The results showed that the levels of PGE₂ productionwere elevated markedly after burns, as compared with the control values （P<0.05～0. 001）, and the effects of significant inhibition of ConA-induced ³H-Tdr incorporationcorrelated with the higher PGE₂ levels （P<0.05～0.001）. A remarkable difference existed between the immunosuppressive effects of the burned and unburned mice’ s peritoneal fluid extracts（P<0.05）. The above suggests that the production of PGE₂ is increasedafter burns, which is able to alter the lymphocyte function. It could be deduced that the elevated PGE₂ production after burns is one of the mediators to the immunosuppression following thermal injury.更多还原